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Construction of Mimic Artificial Blood Vessels with Catalyzing No Generation

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Tutor: WuWenJie
School: Tianjin University of Science and Technology
Course: Applied Chemistry
Keywords: Self-assembly,Bionic,Nitric oxide,Catalytic generation,Artificial vessels
CLC: R318.11
Type: Master's thesis
Year:  2011
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In this paper, the method to fabricate artificial blood vessel was researched to simulate natural vascular structure. Because of the capacity that organic selenium can catalyze endogenous NO donor RSNOs decomposing to release of NO, organic selenium compound was loaded onto the surface of artificial vascular scaffold. The bioactivity of the modified material was evaluated by catalytic generation of nitric oxide, platelet adhesion and kinds of cellular behavior experiments. The feasibility of building implanted vascular scaffold with bionic catalytic controlled generation of NO to obtain anticoagulant property and accelerate endothelialization was further examined.Organic selenium catalyst SePEI were synthesized and characterized, and then used as polycation with PGA as polyanion coated onto the surface of polycaprolactone (PCL) nanofiber scaffolds obtained by electrospinning layer-by-layer. The LBL structures were characterized. Catalytic generation of NO from the NO donors-RSNOs was tested in the existence of reducing agent RSH. Biological properties were also evaluated, such as cell proliferation experiments and anti-coagulation experiments.It can be seen from the infrared spectrum that the amide bond is formed, and there are still a lot of amine groups to ensure water-soluble of the production. The amount of Se element loaded on SePEI is also detected by atomic absorption. The self-assembled layers are characterized qualitative and quantitative by UV-vis and atomic absorption spectrometry. With the increasing of numbers of the layers, Se element content is also added. The laciniation of the contact angle represent the alternating adsorption of polycations and polyanions. It is shown in the catalytic releasing NO test that the level of the release amount of containing Se group is significantly higher than that of control group, indicating that Se has the catalytic function. The NO release was relatively stable with no significant burst appeared, and still could be detected at the80hours. The survival rate of fibroblasts and smooth muscle cells on the surface of the materials are tested. Those confirm that the materials show little cytotoxicity, and have the effect of inhibitting proliferation of smooth muscle. The anticoagulant property of catalyzing NO-releasing grafts (SePEI/PGA)10was characterized by using the AV-shunt experiment. Through the stereo microscope, HE staining and scanning electron microscopy, experiment materials maintain clean and smooth surface and don¡¯t produce acute thrombosis, and the amount of the platelet on the surface of Se containing groups are significantly lower than the control groups. This new style of stent material is capable of catalyzing the NO donor releasing NO. The NO release is relatively stable with no significant burst appeared, and can last for a long time.
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