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DNA-,RNA-binding Behaviors and Cytotoxic Properties of Ru(¢ò) and Co(¢ó) Complexes

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Tutor: TanLiFeng
School: Xiangtan University
Course: Inorganic Chemistry
Keywords: Ruthenium ( II ) complexes,Cobalt ( III ) complexes,Nucleic acid,Cytotoxicity
CLC: O621.1
Type: Master's thesis
Year:  2011
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Abstract:
DNA and RNA is an important component of the organism substances, including many life genetic information, they increasingly demonstrate its irreplaceable role in this field of bio-inorganic chemistry, polypyridyl transition metal complexes such as the nail (II) complexes occupies an important position in the field of photophysical, photochemical studies to study the role of such complexes with nucleic acids to help people study a new type of nucleic acid probe, the nucleic acid molecule optical switch, anticancer drugs; fact of cobalt (III) complexes The material also has the similar nature of the ruthenium (II) complexes, DNA secondary structure can be used as probes or light cleaving agents, based on the synthesis of this thesis describes four polypyridyl complexes, which reads as follows: The first chapter briefly describes the some of the basic information in the field, such as the structure of nucleic acids composition polypyridyl complexes with DNA / RNA role model, research methodology and nail (II) and cobalt (III) polypyridyl complexes with DNA Research topics significance. The second chapter synthesis of two new ruthenium (II) polypyridyl metal complexes [RuIP2 (DPBPD (NH2) 2)] (ClO4) 2 ¡¤ H2O 1, [Ru (IP) 2 (BPIP)] (ClO4) 2 ¡¤ H2O2, by elemental analysis, NMR H spectra and electrospray ionization mass spectrometry (ES-MS) were used to characterize the complex; the use of UV - visible absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), viscosity testing and reverse salt titration test to study the bonding behavior of the complexes 1, 2, and DNA / RNA. Complexes 1 to the classic insert mode with DNA, complex 2 is not fully inserted manner with DNA and with the objects 1, 2, and the role of RNA than DNA stronger; complexes was found 1 the optically selective nucleic acid experiments show that, of the complex with Nucleic affinity interaction stereoselective complexes priority RNA binding; description of the type of nucleic acid can affect its complex three-dimensional sex. Chapters III and IV of the thesis the synthesis and characterization of two novel cobalt (III) polypyridyl metal complexes [Co (phen) 2 BPIP)] (ClO4) 2 ¡¤ H2O 3 [Co (phen) 2PPIP)] ( ClO4) 2 ¡¤ H2O 4, and spectroscopic methods and viscosity test methods to study the interaction of the complexes with DNA / RNA, the results show that the complex 3 is inserted into the mode, and the role of the nucleic acid complexes 4 is Incomplete insertion mode and Nucleic Acids, complexes 3,4 role with RNA than DNA stronger. Also, complexes of 1,3 preliminary cytotoxicity test, the experiment using the MTT assay (3 - (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) (MTT = The results showed that complexes 1,3 on the two types of tumor cells HepG2 (liver cells) and HL-60 (leukemia cells) showed a different anti-tumor activity and expectations of these experiments for broad-spectrum, high efficiency, low toxicity and can be used in clinical anti- cancer drugs the advent provide certain guiding significance.
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